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Clonogenically culturing and identification of liver cancer stem cells (LCSCs). ( A ) Morphologies of three subtypes of LCSCs cultured on MEFs after continuous passages (Top panel) and recoveries (Bottom panel) after the cryopreservation. Scale bar 100 μm. ( B ) The proliferation of ALDH + singe LCSCs cultured on MEFs at days 3, 5 and 7 (Left panel), scale bar 50 μm, and ki67 staining at days 3, 5 and 7 (Left panel). Scale bar 25 μm. The colony formation rate of single LCSCs. n = 3 biological replicates (Right panel). ( C ) Immunofluorescence staining showed that the cultured cloned cells expressed putative LCSC <t>markers</t> <t>(CD44,</t> NCAM, CD133, <t>AFP,</t> SOX2, SOX9, OV6, ALDH1A1, CD24). Scale bar 100 μm. ( D , E ) Determination of the percentages of ALDH positive cells between primary cancer cells and cloned cells. n = 3 biological replicates. Values were represented as mean ± SD. ****p < 0.0001
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Clonogenically culturing and identification of liver cancer stem cells (LCSCs). ( A ) Morphologies of three subtypes of LCSCs cultured on MEFs after continuous passages (Top panel) and recoveries (Bottom panel) after the cryopreservation. Scale bar 100 μm. ( B ) The proliferation of ALDH + singe LCSCs cultured on MEFs at days 3, 5 and 7 (Left panel), scale bar 50 μm, and ki67 staining at days 3, 5 and 7 (Left panel). Scale bar 25 μm. The colony formation rate of single LCSCs. n = 3 biological replicates (Right panel). ( C ) Immunofluorescence staining showed that the cultured cloned cells expressed putative LCSC <t>markers</t> <t>(CD44,</t> NCAM, CD133, <t>AFP,</t> SOX2, SOX9, OV6, ALDH1A1, CD24). Scale bar 100 μm. ( D , E ) Determination of the percentages of ALDH positive cells between primary cancer cells and cloned cells. n = 3 biological replicates. Values were represented as mean ± SD. ****p < 0.0001
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Clonogenically culturing and identification of liver cancer stem cells (LCSCs). ( A ) Morphologies of three subtypes of LCSCs cultured on MEFs after continuous passages (Top panel) and recoveries (Bottom panel) after the cryopreservation. Scale bar 100 μm. ( B ) The proliferation of ALDH + singe LCSCs cultured on MEFs at days 3, 5 and 7 (Left panel), scale bar 50 μm, and ki67 staining at days 3, 5 and 7 (Left panel). Scale bar 25 μm. The colony formation rate of single LCSCs. n = 3 biological replicates (Right panel). ( C ) Immunofluorescence staining showed that the cultured cloned cells expressed putative LCSC markers (CD44, NCAM, CD133, AFP, SOX2, SOX9, OV6, ALDH1A1, CD24). Scale bar 100 μm. ( D , E ) Determination of the percentages of ALDH positive cells between primary cancer cells and cloned cells. n = 3 biological replicates. Values were represented as mean ± SD. ****p < 0.0001

Journal: Journal of Translational Medicine

Article Title: Isolation and identification of patient-derived liver cancer stem cells and development of personalized treatment strategies

doi: 10.1186/s12967-024-05870-9

Figure Lengend Snippet: Clonogenically culturing and identification of liver cancer stem cells (LCSCs). ( A ) Morphologies of three subtypes of LCSCs cultured on MEFs after continuous passages (Top panel) and recoveries (Bottom panel) after the cryopreservation. Scale bar 100 μm. ( B ) The proliferation of ALDH + singe LCSCs cultured on MEFs at days 3, 5 and 7 (Left panel), scale bar 50 μm, and ki67 staining at days 3, 5 and 7 (Left panel). Scale bar 25 μm. The colony formation rate of single LCSCs. n = 3 biological replicates (Right panel). ( C ) Immunofluorescence staining showed that the cultured cloned cells expressed putative LCSC markers (CD44, NCAM, CD133, AFP, SOX2, SOX9, OV6, ALDH1A1, CD24). Scale bar 100 μm. ( D , E ) Determination of the percentages of ALDH positive cells between primary cancer cells and cloned cells. n = 3 biological replicates. Values were represented as mean ± SD. ****p < 0.0001

Article Snippet: Next, the samples were incubated with primary antibodies at 4 °C overnight targeting AFP (MAB1368, R&D system), CD44 (3570 S, Cell Signaling Technology), CD133 (64326 S, Cell Signaling Technology), CD31 (AF23628-SP, R&D system), ALB (ab106582, abcam), NCAM (3576 S, Cell Signaling Technology), SOX2 (4900, Cell Signaling Technology), SOX9 (82630 S, Cell Signaling Technology), CD24 (ab202073, abcam), KI67 (9449, Cell Signaling Technology), OV6 (MAB2020, R&D system), and EPCAM (2929 S, Cell Signaling Technology).

Techniques: Cell Culture, Staining, Immunofluorescence, Clone Assay

Reagent information

Journal: Human Cell

Article Title: Generation of human induced pluripotent stem cell lines derived from patients of cystic biliary atresia

doi: 10.1007/s13577-024-01147-x

Figure Lengend Snippet: Reagent information

Article Snippet: Differentiation marker (Endoderm) , Mouse anti-AFP , 1:200 , R and D Systems, Cat# MAB1368 , RRID:AB_357658.

Techniques: Immunocytochemistry, Cytometry, Marker, Nested PCR, Plasmid Preparation

Reagents and tools table

Journal: EMBO Reports

Article Title: Terminal α1,2-fucosylation of glycosphingolipids by FUT1 is a key regulator in early cell-fate decisions

doi: 10.1038/s44319-024-00243-1

Figure Lengend Snippet: Reagents and tools table

Article Snippet: α-Fetoprotein (αFP) , R&D Systems (mouse, monoclonal) (FACS) , , MAB1368.

Techniques: Immunohistochemistry-Frozen IF, Western Blot, Recombinant, Staining, Plasmid Preparation, Sequencing, Control, Transfection, Over Expression, Software, TaqMan Assay, Expressing, Binding Assay, Marker